Under the conditions of steadily reducing tariffs on agri-food trade, the non-tariff measures (NTMs) can be used to protect national agricultural markets. Such measures are used by both developed countries and developing mainly in relation to agri-food international trade. The main objective of the research was to assess the quantitative use of non-tariff instruments in agri-food import of selected developing countries. The analysis confirmed that non-tariff measures are used in the agri-food imports by developing countries and that within broad group of NTMs the sanitary and phytosanitary measures and the technical barriers mainly applied.