The aim of the paper was to identify factors that can determinate the honey yield of bee colonies in Poland. The research material consisted of the field evaluation results from 495 groups colonies realized in 2011-2014 by the National Animal Breeding Centre (NABC). In the paper was used a method of tripartite analysis of variance. The studies have shown a significant influence of each of the variables (A – year of collecting data, B – province, in which was located apiary, C – bee races) on the honey yield of bee colonies, as well as a significant interaction effect occurring between pairs of variables under consideration.