000211236 001__ 211236
000211236 005__ 20180123002152.0
000211236 0247_ $$2Other$$aISSN 1810-3030
000211236 037__ $$a452-2016-35830
000211236 037__ $$a452-2016-36263
000211236 041__ $$aen_US
000211236 245__ $$aBuffer’s ionic strength on the chaperone-like activity (CLA) of silkworm small heat shock protein: sHSP19.9 and sHSP20.8
000211236 260__ $$c2014-12-31
000211236 269__ $$a2014-12-31
000211236 270__ $$mmthossain@bau.edu.bd$$pHossain,   M. Tofazzal
000211236 300__ $$a9
000211236 336__ $$aJournal Article
000211236 490__ $$a12(2)
000211236 520__ $$aSmall heat-shock proteins (sHSPs), an abundant and ubiquitous family of molecular chaperones, can effectively prevent irreversible aggregation of non-native proteins by forming soluble complex. The CLA of sHSPs is usually determined by the capacity to suppress thermally or chemically induced protein aggregation. Various factors can effectively influence the CLA, and among them the ionic strength of the preparation and working buffer is an important factor. The study deals with the effect of ionic strength of buffer on the CLA of two silkworm sHSPs: namely sHSP19.9 and sHSP20.8 against the thermally-induced aggregation of BLC, a non-native protein. The study clearly revealed that sHSP19.9 required high ionic strength (more NaCl concentration) in reaction buffer to prevent irreversible aggregation of BLC. On the other hand, such high ionic strength condition is not necessary for sHSP20.8 but it influences the activity in some context.
000211236 542__ $$fLicense granted by Md. Atiqur Rahman  Khokon (atiq.ppath@bau.edu.bd) on 2015-10-27T06:07:36Z (GMT):

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000211236 650__ $$aAgricultural and Food Policy
000211236 6531_ $$aIonic strength of buffer
000211236 6531_ $$aCLA
000211236 6531_ $$asilkworm
000211236 6531_ $$asHSP19.9
000211236 6531_ $$asHSP20.8
000211236 700__ $$aHossain, M. Tofazzal
000211236 700__ $$aYoichi Aso
000211236 773__ $$d2015$$jVolume 12$$kNumber 2$$o249$$q241$$tJournal of the Bangladesh Agricultural University
000211236 8564_ $$s374665$$uhttp://ageconsearch.umn.edu/record/211236/files/02.%20JBAU%20531-12.pdf
000211236 887__ $$ahttp://purl.umn.edu/211236
000211236 909CO $$ooai:ageconsearch.umn.edu:211236$$pGLOBAL_SET
000211236 912__ $$nSubmitted by Md. Atiqur Rahman  Khokon (atiq.ppath@bau.edu.bd) on 2015-10-27T06:10:04Z
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  Previous issue date: 2014-12-31
000211236 982__ $$gJournal of the Bangladesh Agricultural University>Volume 12, No. 2, 2015
000211236 980__ $$a452