In order to study high yield cultivation technology for Coix Lachryrma-jobi L., and discuss the effect of planting density, fertilizer level, and number of seedlings per hole on yield of Coix Lachryrma-jobi L., this paper took domesticated variety Xingren Small White Shell of Coix Lachryrma-jobi L. as an example. Using L9(34) orthogonal design, it selected optimum combination of planting density, fertilizer level, and number of seedlings per hole to guide planting of Coix Lachryrma-jobi L.. Experiment indicated that the optimum planting density (spacing in the rows and spacing between rows) of Coix Lachryrma-jobi L. is 40 cm x 80 cm; the optimum fertilizer level is pure nitrogen 15 kg, pure phosphorus 15 kg and pure potassium 15 kg per mu; the optimum number of seedlings per hole is 5 seedlings per hole. Through variance analysis, it indicated that planting density exerts significance effect on the yield of Coix Lachryrma-jobi L.