Presented paper deals with analysis of use of business informatics in agricultural enterprises in the Czech Republic. The source data for the paper were gathered in the survey among agricultural enterprises from various regions of the Czech Republic. There are results of the survey and the overview of the current state of the use of business informatics is described here. Main findings are that it is the owner of the farm who mostly works with business informatics and for most of them the informatics is a necessary technological solution. The investments in farms are currently directed to mobile hardware equipment. Most frequently used programs are accounting information systems, stock control and specialized programs for plant production and livestock.