Based on the survey data of entrepreneurship concerning farmers in China, this article uses the multivariate adjustment regression analysis method, to analyze the factors influencing farmers' identification of entrepreneurial opportunity and the mechanism. The results show that demographic characteristics are still an important factor influencing farmers' identification of entrepreneurial opportunity, but the extent of its influence is weaker than entrepreneurs' trait. The new trait theory is verified in farmers' entrepreneurship opportunity behavior; entrepreneurship environment is becoming an important factor influencing entrepreneurial opportunity identification, whose regulation effect on entrepreneurs' social network and previous experience is stronger than the regulation effect on entrepreneurs' psychological trait.