On the basis of AHP method, this paper establishes comprehensive evaluation model of demonstration farmers' specialized cooperatives, uses the Delphi method and AHP software to assign scientific and objective weight to 7 criteria in criteria layer and 27 indices in index layer, and evaluate the overall level of 153 farmers' specialized cooperatives whose main business is fruits and vegetables in Region R. The results show that the overall operation level of farmers' specialized cooperatives is common; democratic and financial management is open; the information-based level and social sensation are not insufficient; the marking results of questionnaire is basically scientific and objective. This paper discusses the new way to evaluate operation level of farmers' specialized cooperatives, and puts forward relevant countermeasures and suggestions as follows: promote me information-based level of demonstration cooperatives; strengthen the service capacity of demonstration cooperatives; use means of science and technology to promote product quality; amplify social sensation of farmers' specialized cooperatives.