Taking Lingtong Grand Canyon in Jingxi County, Baise City, Guangxi, China as a research object, the face-to-face questionnaire survey on tourist is carried out, in order to obtain the basic situation of the interviewees, as well as their expectation for the eco-tourism experience. Factor analysis method is adopted to test the interviewees’ expectation of the eco-tourism experience. Result shows that eco-tourism experience of tourist is mainly reflected in the ecological leisure experience, the ecological tourism experience, the ecological experience in scenic spot, and the self-realization experience. Analysis on the recognition of the expectation for eco-tourism experience shows that the interviewees’ expectations of the eco-tourism experience are in the order of the ecological experience in scenic spot, the eco-tourism experience, the ecological leisure experience, and the self-realization experience. Difference analysis on the interviewees’ expectations for the eco-tourism experience is carried out by market segmentation. Result shows that there are significant differences in the demand of eco-tourism experience among different types of tourisms. According to the investigation result, countermeasures for the construction and marketing of eco-tourism sites are put forward.