The sugar industry in Queensland (Australia) is confronted with increasing economic pressure and environmental constraints. To explore whether agri-industrial diversification of the sugar industry provides a sustainable development pathway for the region, we develop a spatial environmental-economic approach that integrates a land use and value chain model with a hydrological model. Results indicate that agri-industrial diversification can lead to substantial increases in regional income, while at the same time increasing the resilience of a sugar industry facing decreasing sugar prices. Agri-industrial diversification drives land use diversification, which under current sugar prices does not lead to a reduction in sugarcane production. Water quality benefits from this land use diversification are mixed, and depend on the economic viability and erosion characteristics of the concerned production systems.