In order to establish a quick and specific method which could identify the avian-derived ingredients, this study used 16S rRNA gene sequence as target site, and designed the specific primers of chicken, pigeon meat and quail meat. The DNA of common livestock and poultry meat (including mutton, beef, pork, rabbit meat, pigeon meat, quail meat, chicken, duck and goose) was used as template. Though PCR amplification and specific detection, a quick determination method was established to identify the avian-derived ingredients. The results showed that the selected primers could identify the ingredients of animal origin effectively and quickly. The method was convenient and concise, and could detect the chicken-derived, pigeon -derived, quail-derived ingredients in livestock and poultry food quickly and accurately.