The cultural construction in agricultural enterprises in China is not so optimistic especially compared with developed countries. In 2015, as the Party Central Committee in China put forward strategy of building a moderately prosperous society in all aspects, it is necessary for Chinese agricultural enterprise to gain implications for cultural construction by studying Japanese culture. Japanese culture features “group spirit, interpersonal relation, absorbing advanced culture and rational spirit” as its quintessence and with “lifetime employment, annual merits, decision making on application, cooperative management”. Chinese agricultural enterprises should find ways for cultural construction which is beneficial for enhancing staff awareness and management from quintessence and outward manifestation of Japanese culture. By doing so, it will promote the cultural construction of Chinese agricultural enterprises and lift the core-competitiveness.