Through a series of exploration based on PSR framework model, for the purpose of building a suitable Chongqing agricultural non-point source pollution evaluation index system model framework, combined with the presence of Chongqing specific agro-environmental issues, we build a agricultural non-point source pollution assessment index system, and then study the agricultural system pressure, agro-environmental status and human response in total 3 major categories, develope an agricultural non-point source pollution evaluation index consisting of 3 criteria indicators and 19 indicators. As can be seen from the analysis, pressures and responses tend to increase and decrease linearly, state and complex have large fluctuations, and their fluctuations are similar mainly due to the elimination of pressures and impact, increasing the impact for agricultural non-point source pollution.